Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection.
Author(s)
Garson, Jeremy A
Kellam, Paul
Towers, Greg J
Type
Journal Article
Abstract
XMRV is a gammaretrovirus associated in some studies with human prostate cancer and chronic fatigue syndrome. Central to the hypothesis of XMRV as a human pathogen is the description of integration sites in DNA from prostate tumour tissues. Here we demonstrate that 2 of 14 patient-derived sites are identical to sites cloned in the same laboratory from experimentally infected DU145 cells. Identical integration sites have never previously been described in any retrovirus infection. We propose that the patient-derived sites are the result of PCR contamination. This observation further undermines the notion that XMRV is a genuine human pathogen.
Date Issued
2011-02-25
Date Acceptance
2011-02-25
Citation
Retrovirology, 2011, 8 (13), pp.13-13
ISSN
1742-4690
Publisher
BioMed Central
Start Page
13
End Page
13
Journal / Book Title
Retrovirology
Volume
8
Issue
13
Copyright Statement
© 2011 Garson et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/21352548
PII: 1742-4690-8-13
Subjects
Base Sequence
Cell Line, Tumor
DNA Contamination
DNA, Viral
Humans
Male
Molecular Sequence Data
Polymerase Chain Reaction
Prostate
Prostatic Neoplasms
Virus Integration
Xenotropic murine leukemia virus-related virus
Publication Status
Published online
Coverage Spatial
England