Impact of fructooligosaccharides on gut microbiota and microbial metabolites in gestational diabetes mellitus: an in vitro fermentation model study
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Gestational diabetes mellitus (GDM) is a prevalent metabolic disorder linked to gut microbiota dysbiosis. This study investigates the effects of fructooligosaccharides (FOS) on gut microbiota, microbial metabolites, and functional pathways using an in vitro fermentation model. Fecal samples from ten GDM patients were treated with FOS and analyzed via 16S rRNA sequencing and metabolomics. FOS supplementation enriched beneficial genera (Bifidobacterium, Lactobacillus, Roseburia) while reducing harmful taxa (Escherichia-Shigella). Metabolically, it increased acetate production and lowered harmful gases (ammonia, hydrogen sulfide). Predictive functional analysis revealed enhanced ribosome activity, amino acid biosynthesis, and quorum sensing, alongside reduced sugar metabolism and ATP-binding cassette transporters. These findings highlight FOS as a promising prebiotic for modulating gut microbiota, boosting short-chain fatty acid production, and improving metabolic health in GDM patients. This study offers novel insights into the mechanisms by which FOS influences gut microbial ecology and its therapeutic potential for GDM management.
Date Issued
2025-04-01
Date Acceptance
2025-03-13
Citation
Journal of Functional Foods, 2025, 127
ISSN
1756-4646
Publisher
Elsevier
Journal / Book Title
Journal of Functional Foods
Volume
127
Copyright Statement
1756-4646/© 2025 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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Publication Status
Published
Article Number
106740
Date Publish Online
2025-03-23