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Single-channel digital LAMP multiplexing using Amplification Curve Analysis
File(s)
d2sd00038e.pdf (1.37 MB)
Published version
OA Location
Author(s)
Malpartida-Cardenas, Kenny
Miglietta, Luca
Peng, Tianyi
Moniri, Ahmad
Holmes, Alison
Type
Journal Article
Abstract
Loop-mediated isothermal amplification assays are currently limited to one target per reaction in the absence of melting curve analysis, molecular probes or restriction enzyme digestion. Here, we demonstrate multiplexing of five targets in a single fluorescent channel using digital LAMP and the machine learning-based method amplification curve analysis, resulting in a classification accuracy of 91.33% on 54 186 positive amplification events.
Date Issued
2022-04-11
Date Acceptance
2022-04-09
Citation
Sensors and Diagnostics, 2022, 1 (1), pp.465-468
URI
URL
DOI
ISSN
2635-0998
Publisher
Royal Society of Chemistry (RSC)
Start Page
465
End Page
468
Journal / Book Title
Sensors and Diagnostics
Volume
1
Issue
1
Copyright Statement
© 2022 The Author(s). Published by the Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
License URL
Sponsor
Imperial College Healthcare NHS Trust- BRC Funding
Imperial College COVID-19 Research Fund
Identifier
https://pubs.rsc.org/en/content/articlelanding/2022/SD/D2SD00038E
Grant Number
RDA02
Publication Status
Published
Date Publish Online
2022-04-11