Basophils, high-affinity IgE receptors and CCL2 in human anaphylaxis
File(s)
Published version
Accepted version
Author(s)
Turner, PJ
custovic, A
Shamji, MH
Type
Journal Article
Abstract
Background
:
The role of basophils in anaphylaxis is
un
clear
.
Objective
:
T
o
investigate
whether
basophils
have
an
important
role
in human anaphylaxis
.
Methods
:
In
an
emergency department study, w
e
recruited 31
patients
with
acute
anaphylaxis
,
predominantly to
hymenoptera
venom. We
measured
expression of
basophil
activation markers
(
CD63
, CD203c)
,
the
absolute number of circulating basophils,
whole
-
blood
FcεRI
,
CPA3
and
HDC
gene
expression
,
and
serum
markers
(
CCL2
,
CCL5,
CCL11,
IL
-
3
,
TSLP
)
at
three
time points
(
during
the
anaphylactic episode
, and in
convalescent
samples
7
and 30
day
s
later
).
We
recruited
134
h
ymenoptera
-
allergic
and
76
healthy controls
for comparison
. We
then
investigated whether the changes
observed
during venom
-
related
anaphylaxis
also
occur during allergic reactions
to food
in 22 peanut
-
allergic
individuals
undergoing double
-
blind placebo
-
controlled
food challenge to
peanut
(DBPC
F
C).
Results
:
The number of circulating basophils was significantly lower during
anaphylaxis
(median 3.5 cells/μl)
than
7
and
30
days
later
(17.5
and
24.7 cells/μl
, P<0.0001
)
, and
compared to
venom
-
allerg
ic
and healthy controls (21
and
23.4 cells/μl
,
P
<0.0001
)
.
FcεRI
expression
during
anaphylaxis
w
as
also
significantly
lower than
in convalescent samples
(
P≤
0.00
2) and venom
-
allerg
ic
control
s
(
P
<0.0001
).
CCL2
(but not other serum markers)
was
significant
ly
higher
during
anaphylaxis
(median 658 pg/ml)
than in convalescent samples
(314
and 311
pg/ml
,
7 and 30
days
,
P<0.001).
Peanut
-
induced allergic reactions resulted in
a
significant decrease in
circulating basop
hils
compared to pre
-
challenge sample
s
(P=0.016), a
decrease in
FcεRI
expression (P=0.007)
, and a
n
increase in
CCL2
(P=0.003)
.
Conclusions
:
O
ur
findings
imply
an important and specific role
for
basophils in the
pathophysiology of human anaphylaxis.
:
The role of basophils in anaphylaxis is
un
clear
.
Objective
:
T
o
investigate
whether
basophils
have
an
important
role
in human anaphylaxis
.
Methods
:
In
an
emergency department study, w
e
recruited 31
patients
with
acute
anaphylaxis
,
predominantly to
hymenoptera
venom. We
measured
expression of
basophil
activation markers
(
CD63
, CD203c)
,
the
absolute number of circulating basophils,
whole
-
blood
FcεRI
,
CPA3
and
HDC
gene
expression
,
and
serum
markers
(
CCL2
,
CCL5,
CCL11,
IL
-
3
,
TSLP
)
at
three
time points
(
during
the
anaphylactic episode
, and in
convalescent
samples
7
and 30
day
s
later
).
We
recruited
134
h
ymenoptera
-
allergic
and
76
healthy controls
for comparison
. We
then
investigated whether the changes
observed
during venom
-
related
anaphylaxis
also
occur during allergic reactions
to food
in 22 peanut
-
allergic
individuals
undergoing double
-
blind placebo
-
controlled
food challenge to
peanut
(DBPC
F
C).
Results
:
The number of circulating basophils was significantly lower during
anaphylaxis
(median 3.5 cells/μl)
than
7
and
30
days
later
(17.5
and
24.7 cells/μl
, P<0.0001
)
, and
compared to
venom
-
allerg
ic
and healthy controls (21
and
23.4 cells/μl
,
P
<0.0001
)
.
FcεRI
expression
during
anaphylaxis
w
as
also
significantly
lower than
in convalescent samples
(
P≤
0.00
2) and venom
-
allerg
ic
control
s
(
P
<0.0001
).
CCL2
(but not other serum markers)
was
significant
ly
higher
during
anaphylaxis
(median 658 pg/ml)
than in convalescent samples
(314
and 311
pg/ml
,
7 and 30
days
,
P<0.001).
Peanut
-
induced allergic reactions resulted in
a
significant decrease in
circulating basop
hils
compared to pre
-
challenge sample
s
(P=0.016), a
decrease in
FcεRI
expression (P=0.007)
, and a
n
increase in
CCL2
(P=0.003)
.
Conclusions
:
O
ur
findings
imply
an important and specific role
for
basophils in the
pathophysiology of human anaphylaxis.
Date Issued
2017-03-22
Date Acceptance
2016-12-23
Citation
Journal of Allergy and Clinical Immunology, 2017, 140 (3), pp.750-758.e15
ISSN
1097-6825
Publisher
Elsevier
Start Page
750
End Page
758.e15
Journal / Book Title
Journal of Allergy and Clinical Immunology
Volume
140
Issue
3
Copyright Statement
2017 The Authors. Published by Elsevier Inc. on behalf of the American Academy of
Allergy, Asthma & Immunology. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/).
Allergy, Asthma & Immunology. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/).
License URL
Sponsor
Medical Research Council (MRC)
National Institute for Health Research
Grant Number
MR/K010468/1
RDD02
Subjects
Science & Technology
Life Sciences & Biomedicine
Allergy
Immunology
Anaphylaxis
basophils
CD63 activation
Fc epsilon RI expression
CCL2
serum tryptase
PLATELET-ACTIVATING-FACTOR
INSECT-STING CHALLENGE
ALLERGIC REACTIONS
MAST-CELL
ATOPIC-DERMATITIS
FOOD CHALLENGES
TRYPTASE LEVELS
TRANSENDOTHELIAL MIGRATION
VENOM IMMUNOTHERAPY
IMMUNOGLOBULIN-E
FcεRI expression
Adolescent
Adult
Aged
Animals
Basophils
Cytokines
Double-Blind Method
Female
Gene Expression
Humans
Hymenoptera
Leukocyte Count
Male
Middle Aged
Peanut Hypersensitivity
Receptors, IgE
Venoms
Young Adult
1107 Immunology
Publication Status
Published