A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing.
Author(s)
Type
Journal Article
Abstract
Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral contigs that can be generated by de novo assembly. This in turn enables the Illumina next generation sequencing (NGS) platform to be used as an effective tool for viral discovery from tissue samples.
Date Issued
2011-12-21
Date Acceptance
2011-11-16
Citation
PLoS One, 2011, 6 (12), pp.e28879-e28879
ISSN
1932-6203
Publisher
Public Library of Science
Start Page
e28879
End Page
e28879
Journal / Book Title
PLoS One
Volume
6
Issue
12
Copyright Statement
© 2011 Daly et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/22216131
PII: PONE-D-11-16643
Subjects
Animals
Biopsy
Dogs
Humans
Liver
RNA, Viral
Viruses
Publication Status
Published
Coverage Spatial
United States
Article Number
e28879