Cancer Cell Discrimination Using Host-Guest "Doubled" Arrays
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
We report a nanosensor that uses cell lysates to rapidly profile the tumorigenicity of cancer cells. This sensing platform uses host–guest interactions between cucurbit[7]uril and the cationic headgroup of a gold nanoparticle to non-covalently modify the binding of three fluorescent proteins of a multi-channel sensor in situ. This approach doubles the number of output channels to six, providing single-well identification of cell lysates with 100% accuracy. Significantly, this classification could be extended beyond the training set, determining the invasiveness of novel cell lines. The unique fingerprint of these cell lysates required minimal sample quantity (200 ng, ∼1000 cells), making the methodology compatible with microbiopsy technology.
Date Issued
2017-05-23
Date Acceptance
2017-05-01
Citation
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2017, 139 (23), pp.8008-8012
ISSN
0002-7863
Publisher
American Chemical Society
Start Page
8008
End Page
8012
Journal / Book Title
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume
139
Issue
23
Copyright Statement
© 2017 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://dx.doi.org/10.1021/jacs.7b03657
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000403631200048&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
SUPRAMOLECULAR NANOPARTICLES
MOLECULAR RECOGNITION
FLUORESCENT PROTEIN
SYNTHETIC RECEPTOR
METASTATIC CELLS
CHEMICAL NOSE
SENSOR ARRAY
AMINO-ACIDS
IN-VIVO
TURN-ON
03 Chemical Sciences
General Chemistry
Publication Status
Published