Adaptive Multiphoton Endomicroscope Incorporating a Polarization-Maintaining Multicore Optical Fibre
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Accepted version
Published version
Author(s)
Type
Journal Article
Abstract
We present a laser scanning multiphoton endomicroscope
with no distal optics or mechanical components that incorporates
a polarization-maintaining (PM) multicore optical fibre to
deliver, focus, and scan ultrashort pulsed radiation for two-photon
excited fluorescence imaging. We show theoretically that the use of
a PM multicore fibre in our experimental configuration enhances
the fluorescence excitation intensity achieved in the focal spot compared
to a non-PM optical fibre with the same geometry and con-
firm this by computer simulations based on numerical wavefront
propagation. In our experimental system, a spatial light modulator
(SLM) is utilised to program the phase of the light input to each of
the cores of the endoscope fibre such that the radiation emerging
from the distal end of the fibre interferes to provide the focused
scanning excitation beam. We demonstrate that the SLM can enable
dynamic phase correction of path-length variations across the
multicore optical fibre whilst the fibre is perturbed with an update
rate of 100 Hz.
with no distal optics or mechanical components that incorporates
a polarization-maintaining (PM) multicore optical fibre to
deliver, focus, and scan ultrashort pulsed radiation for two-photon
excited fluorescence imaging. We show theoretically that the use of
a PM multicore fibre in our experimental configuration enhances
the fluorescence excitation intensity achieved in the focal spot compared
to a non-PM optical fibre with the same geometry and con-
firm this by computer simulations based on numerical wavefront
propagation. In our experimental system, a spatial light modulator
(SLM) is utilised to program the phase of the light input to each of
the cores of the endoscope fibre such that the radiation emerging
from the distal end of the fibre interferes to provide the focused
scanning excitation beam. We demonstrate that the SLM can enable
dynamic phase correction of path-length variations across the
multicore optical fibre whilst the fibre is perturbed with an update
rate of 100 Hz.
Date Issued
2016-05-01
Date Acceptance
2015-09-29
Citation
IEEE Journal of Selected Topics in Quantum Electronics, 2016, 22 (3)
ISSN
1558-4542
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Journal / Book Title
IEEE Journal of Selected Topics in Quantum Electronics
Volume
22
Issue
3
Copyright Statement
This work is licensed under a Creative Commons Attribution 3.0 License. For more information, see http://creativecommons.org/licenses/by/3.0/
License URL
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Grant Number
EP/K020102/1
Subjects
Science & Technology
Technology
Physical Sciences
Engineering, Electrical & Electronic
Optics
Physics, Applied
Engineering
Physics
Adaptive optics
multiphoton endomicroscope
polarization-maintaining multicore optical fibre
wavefront shaping
FLUORESCENCE MICROSCOPY
2-PHOTON MICROSCOPE
LENSLESS ENDOSCOPE
SCATTERING MEDIA
MULTIMODE FIBER
HIGH-RESOLUTION
COMPACT
EXCITATION
MIRROR
PULSES
Article Number
6800708