SiO₂-CAOCME/poly(tetrahydrofuran)/poly(caprolactone) 3D-printed scaffolds drive human-bone marrow stromal cell osteogenic differentiation
Author(s)
Sory, David R
Heyraud, Agathe CM
Jones, Julian R
Rankin, Sara M
Type
Journal Article
Abstract
This article addresses the unmet clinical need of scaffolds for bone regeneration that can combine osteogenic properties, such as the promotion of bone marrow stem cell differentiation into osteoblasts, with the ability to withstand cyclic loading. In our previous study, we demonstrated that discs of SiO2-CaOCME/poly(tetrahydrofuran)/poly(caprolactone) hybrids or their dissolution products can drive terminal osteogenic differentiation of human bone marrow stromal cells (h-BMSCs) in vitro. The current study shows that the 3D-printed hybrid scaffolds with physiologically relevant 3D architecture further promote h-BMSC osteogenesis. The 3D-printed scaffolds support spatially organized cell behavior in an environment mirroring conditions relevant to off-the-shelf implant applications. Primary cellular functions, including viability, adhesion, and proliferation, were maintained across 3D scaffold surfaces and within inter-strut regions. osteogenic commitment was evidenced by the upregulation of lineage-specific transcripts, hydroxyapatite deposition, and the organized assembly of extracellular matrix (ECM) proteins. Our results demonstrate that 3D-printed scaffolds drive osteogenesis by modulating cell metabolism, inducing osteogenic morphological transitions, and promoting the expression of osteocalcin and collagen type I alpha 1 chain, alongside hydroxyapatite matrix mineralization. Collectively, our findings highlight the SiO2-CaOCME/poly(tetrahydrofuran)/poly(caprolactone) scaffold's strong osteogenic properties—driven by composition, surface architecture, and ion release – and its promise for clinical bone regeneration.
Date Issued
2026-05-08
Date Acceptance
2025-12-19
Citation
Advanced Healthcare Materials, 2026, 15 (17)
ISSN
2192-2640
Publisher
Wiley
Journal / Book Title
Advanced Healthcare Materials
Volume
15
Issue
17
Copyright Statement
© 2026 The Author(s)AdvanceHealthcarMaterials published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/41630141
Subjects
bioactive inorganic/organic hybrids
bone tissue engineering
human‐bone marrow stromal cells
osteogenic differentiation
sol‐gel
Humans
Osteogenesis
Tissue Scaffolds
Polyesters
Mesenchymal Stem Cells
Cell Differentiation
Printing, Three-Dimensional
Furans
Silicon Dioxide
Cells, Cultured
Cell Proliferation
Tissue Engineering
Butylene Glycols
Polymers
Publication Status
Published
Coverage Spatial
Germany
Article Number
e03733
Date Publish Online
2026-02-02