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Identification of commonly expressed exoproteins and proteolytic cleavage events by proteomic mining of clinically-relevant UK isolates of Staphylococcus aureus
File(s)
mgen000049.pdf (1.72 MB)
Published version
Author(s)
Smith,, DS
Siggins,, MK
Gierula,, M
Pichon,, B
Turner,, CE
Type
Journal Article
Abstract
The range of exoproteins and core exoproteome of 14 S. aureus isolates representing major lineages associated with asymptomatic carriage and clinical disease in the United Kingdom was identified by mass spectrometry proteomics using a combined database incorporating sequences derived from 39 S. aureus genomes. In all, 632 different proteins were identified and, of these, only 52 (8%) were found in all 14 isolates whereas 144 (23%) were found in just a single isolate. Comparison of the observed mass of each protein (based on migration by SDS-polyacrylamide electrophoresis) with its predicted mass (based on amino acid sequence) suggested that 95% of the proteins identified were not subject to any major post translational modification. Migration of 5% of proteins was not as expected: 1% of proteins migrated at a mass greater than predicted, while 4% of proteins appeared to have undergone proteolytic cleavage; these included SsaA2, Aur, SspP, Ebh as well as BlaR1, MecR1, FsH, OatA and LtaS. Intriguingly, a truncated SasG was produced by a single CC8 USA300-like strain. The analysis provided evidence of the marked heterogeneity in protein expression by S. aureus in broth, while yielding a core but narrow common exoproteome.
Date Issued
2016-02-09
Date Acceptance
2016-01-11
Citation
Microbial Genomics, 2016, 2 (2)
URI
DOI
ISSN
2057-5858
Publisher
Microbiology Society
Journal / Book Title
Microbial Genomics
Volume
2
Issue
2
Copyright Statement
© 2016 The Authors. Published by Microbiology Society. This is an open access article published by the Microbiology Society under the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/)
License URL
Sponsor
Wellcome Trust
Medical Research Council (MRC)
Medical Research Council (MRC)
Imperial College Healthcare NHS Trust- BRC Funding
Grant Number
087732/Z/08/Z
G0800777
G0800777
RD009
Publication Status
Published