Optimized sample handling minimizes peptide adsorption to plastics to enable high sensitivity evosep based chemical proteomics
File(s)
Author(s)
Type
Journal Article
Abstract
Chemical proteomics approaches often yield low peptide recovery because they aim to enrich low-abundance proteins. Poor sample handling further reduces recovery through peptide adsorption to plastic surfaces and losses due to vacuum evaporation. We systematically mapped these losses across buffers, volumes, plastics and pH, then developed an Evotip-compatible handling protocol that minimizes adsorption and removes the need for vacuum evaporation. Losses to plastic adsorption and vacuum evaporation were most apparent at low inputs (< 200 ng) and with larger volumes (> 20 µL) but were also dependent on acidification as well as the buffer and plastic used. The optimized workflow: direct acidification of peptides, frozen storage if needed, and direct loading onto Evotips, resulted in up to ∼90-fold gains versus workflows incorporating vacuum concentration steps, a common practice in proteomics sample preparation, when peptide input was limited to 10 ng. This optimized sample handling method enables high-throughput chemical proteomics by reducing manual handling steps and enables the characterization of low abundance proteins previously lost during sample preparation.
Date Issued
2026-06-22
Date Acceptance
2026-06-04
Citation
Proteomics, 2026
ISSN
1615-9853
Publisher
Wiley
Journal / Book Title
Proteomics
Copyright Statement
© 2026 The Author(s). Proteomics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/42328708
Publication Status
Published online
Coverage Spatial
Germany
Article Number
e70154
Date Publish Online
2026-06-22