A novel immunoassay for malondialdehyde-conjugated low-density lipoprotein measures dynamic changes in the blood of patients undergoing coronary artery bypass graft surgery
File(s)
Published version
Author(s)
Hartley, Adam
Khamis, Ramzi
Type
Journal Article
Abstract
Oxidized low-density lipoproteins play an important role in tissue pathology. In this study, we report a sensitive novel enzyme-linked immunosorbent assay for the detection of malondial-dehyde-modified low-density lipoprotein (MDA-LDL), a key component of oxidized LDL. The assay is capable of measuring a variable presence of MDA-LDL within human plasma and serum. We demonstrate the robust nature of the assay on samples stored for over 20 months, as well as high inter-operator reproducibility (r=0.74, p <0.0001). The assay was capable of detecting dynamic changes in patient blood samples after coronary artery bypass graft surgery, indicating synthesis or release of MDA-LDL with the oxidative stress of surgery, followed by homeostatic clearance. This robust, sensitive and specific assay for circulating MDA-LDL will serve as a valuable trans-lational tool for the improved detection of oxidative forms of LDL in response to a range of physiological or pathological stimuli, with potential clinical applicability.
Date Issued
2021-08-17
Date Acceptance
2021-08-10
Citation
Antioxidants, 2021, 10 (8), pp.1-14
ISSN
2076-3921
Publisher
MDPI AG
Start Page
1
End Page
14
Journal / Book Title
Antioxidants
Volume
10
Issue
8
Copyright Statement
© 2021 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
License URL
Sponsor
Wellcome Trust
British Heart Foundation
National Institute for Health Research
Imperial Health Charity
Wellcome Trust
Imperial College Healthcare NHS Trust- BRC Funding
Imperial College Healthcare NHS Trust
Identifier
https://www.mdpi.com/2076-3921/10/8/1298
Grant Number
FS/17/16/32560
RDB02 79560
5118
203928/Z/16/Z
RDB02
PR010 74250
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Chemistry, Medicinal
Food Science & Technology
Pharmacology & Pharmacy
atherosclerosis
low-density lipoprotein (LDL)
oxidized LDL
malondialdehyde
anti-oxidized LDL antibodies
antibodies
assay
inflammation
CIRCULATING OXIDIZED LDL
MARKER
anti-oxidized LDL antibodies
antibodies
assay
atherosclerosis
inflammation
low-density lipoprotein (LDL)
malondialdehyde
oxidized LDL
Publication Status
Published
Date Publish Online
2021-08-17