Quantitative expression of key cancer markers in the AS-30D hepatocarcinoma model
File(s)
Published version
Author(s)
Briones Orta, Marco
Delgado-Coello, Blanca
Roxana Gutiérrez-Vidal, Roxana
Sosa-Garrocho, Marcela
Macias-Silva, Marina
Type
Journal Article
Abstract
Hepatocellular carcinoma is one of the cancers with the highest mortality rate worldwide. HCC is often diagnosed when the disease is already in an advanced stage, making the discovery and implementation of biomarkers for the disease a critical aim in cancer research. In this study, we aim to quantify the transcript levels of key signaling molecules relevant to different pathways known to participate in tumorigenesis, with special emphasis on those related to cancer hallmarks and epithelial-mesenchymal transition, using as a model the murine transplantable hepatocarcinoma AS30 30D. Using qPCR to quantify the mRNA levels of genes involved in tumorigenesis, we found elevated levels for Tgfb1 and Spp1, two master regulators of EMT. A mesenchymal signature profile for AS-30D cells is also supported by the overexpression of genes encoding for molecules known to be associated to aggressiveness and metastatic phenotypes such as Foxm1, C-met, and Inppl1. This study supports the use of the AS-30D cells as an efficient and cost-effective model to study gene expression changes in HCC, especially those associated with the EMT process.
Date Issued
2021-10
Date Acceptance
2021-09-14
Citation
Frontiers in Oncology, 2021, 11, pp.1-11
ISSN
2234-943X
Publisher
Frontiers Media
Start Page
1
End Page
11
Journal / Book Title
Frontiers in Oncology
Volume
11
Copyright Statement
© 2021 Briones-Orta, Delgado-Coello, Gutiérrez-Vidal, Sosa-Garrocho, Macías-Silva and Mas-Oliva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Identifier
https://www.frontiersin.org/articles/10.3389/fonc.2021.670292/full
Subjects
1112 Oncology and Carcinogenesis
Publication Status
Published
Article Number
670292
Date Publish Online
2021-10-19