Balancing the fluorescence imaging budget for all-optical neurophysiology experiments
File(s)
Accepted version
Author(s)
Quicke, Peter
Howe, Carmel L
Foust, Amanda
Type
Chapter
Abstract
The goal of this chapter is to establish a framework to evaluate imaging methodologies for all-optical neurophysiology experiments. This is not an exhaustive review of fluorescent indicators and imaging modalities but rather aims to distill the functional imaging principles driving the choice of both. Scientific priorities determine whether the imaging strategy is based on an “optimal fluorescent indicator” or “optimal imaging modality.” The choice of the first constrains the choice of the second due to each’s contributions to the fluorescence budget and signal-to-noise ratio of time-varying fluorescence changes.
Editor(s)
Papagiakoumou, Eirini
Date Issued
2021-02-12
Citation
All-optical methods to study neuronal function, 2021
Publisher
Humana Press
Journal / Book Title
All-optical methods to study neuronal function
Neuromethods
Copyright Statement
Copyright reserved
Sponsor
Royal Academy Of Engineering
Biotechnology and Biological Sciences Research Council (BBSRC)
Engineering and Physical Sciences Research Council
Grant Number
RF1415\14\26
BB/R009007/1
EP/R512540/1
Publication Status
Accepted