Aza-macrocyclic triphenylamine ligands for G-quadruplex recognition
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Supporting information
Accepted version
Author(s)
Type
Journal Article
Abstract
A new series of triphenylamine-based ligands with one (TPA1PY), two (TPA2PY) or three pending aza-macrocycle(s) (TPA3PY) have been synthesised and studied by means of pH-metric titrations, UV/Vis spectroscopy and fluorescence experiments. The affinity of these ligands for G-quadruplex (G4) DNA and its selectivity over duplex DNA were investigated by FRET melting assays, fluorimetric titrations and circular dichroism (CD) spectroscopy. Interestingly, the interaction of the bi- and specially the tri-branched ligand with G4 leads to a very intense red-shifted fluorescence emission band which may be associated with intermolecular aggregation between the molecule and the DNA. This light-up effect allows the application of the ligands as fluorescence probes to selectivity detect G4.
Date Issued
2018-07-25
Date Acceptance
2018-05-16
Citation
Chemistry - A European Journal, 2018, 24 (42), pp.10850-10858
ISSN
0947-6539
Publisher
Wiley
Start Page
10850
End Page
10858
Journal / Book Title
Chemistry - A European Journal
Volume
24
Issue
42
Copyright Statement
© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the pre-peer reviewed version of the following article, which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201802077.
Sponsor
The Royal Society
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/29768679
Grant Number
NF140726
Subjects
DNA
G-quadruplex
aggregation-induced emission
fluorescent probes
triphenylamine polyamines
Publication Status
Published
Coverage Spatial
Germany
Date Publish Online
2018-06-27