A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair
File(s)
Accepted version
Author(s)
Cheng, Kaiying
Wilkinson, Martin
Chaban, Yuriy
Wigley, Dale B
Type
Journal Article
Abstract
The RecBCD complex plays key roles in phage DNA degradation, CRISPR array acquisition (adaptation) and host DNA repair. The switch between these roles is regulated by a DNA sequence called Chi. We report cryo-EM structures of the Escherichia coli RecBCD complex bound to several different DNA forks containing a Chi sequence, including one in which Chi is recognized and others in which it is not. The Chi-recognized structure shows conformational changes in regions of the protein that contact Chi and reveals a tortuous path taken by the DNA. Sequence specificity arises from interactions with both the RecC subunit and the sequence itself. These structures provide molecular details for how Chi is recognized and insights into the changes that occur in response to Chi binding that switch RecBCD from bacteriophage destruction and CRISPR spacer acquisition to constructive host DNA repair.
Date Issued
2020-01-06
Date Acceptance
2019-11-21
Citation
Nature Structural and Molecular Biology, 2020, 27 (1), pp.71-77
ISSN
1545-9985
Publisher
Nature Research
Start Page
71
End Page
77
Journal / Book Title
Nature Structural and Molecular Biology
Volume
27
Issue
1
Copyright Statement
© 2020 Springer Nature Limited.
Sponsor
Cancer Research UK
Medical Research Council (MRC)
Wellcome Trust
Wellcome Trust
Wellcome Trust
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/31907455
PII: 10.1038/s41594-019-0355-2
Grant Number
12799
MR/N009258/1
202926/Z/16/Z
206175/Z/17/Z
212938/Z/18/Z
Subjects
11 Medical and Health Sciences
03 Chemical Sciences
06 Biological Sciences
Biophysics
Developmental Biology
Publication Status
Published
Coverage Spatial
United States