Activity- and reactivity-based proteomics: Recent technological advances and applications in drug discovery.
File(s)
Accepted version
Author(s)
Benns, Henry James
Wincott, Ceire Jane
Tate, Edward William
Child, Matthew Andrew
Type
Journal Article
Abstract
Activity-based protein profiling (ABPP) is recognized as a powerful and versatile chemoproteomic technology in drug discovery. Central to ABPP is the use of activity-based probes to report the activity of specific enzymes or reactivity of amino acid types in complex biological systems. Over the last two decades, ABPP has facilitated the identification of new drug targets and discovery of lead compounds in human and infectious disease. Furthermore, as part of a sustained global effort to illuminate the druggable proteome, the repertoire of target classes addressable with activity-based probes has vastly expanded in recent years. Here, we provide an overview of ABPP and summarise the major technological advances with an emphasis on probe development.
Date Issued
2020-08-05
Date Acceptance
2020-06-22
Citation
Current Opinion in Chemical Biology, 2020, 60, pp.20-29
ISSN
1367-5931
Publisher
Elsevier
Start Page
20
End Page
29
Journal / Book Title
Current Opinion in Chemical Biology
Volume
60
Copyright Statement
© 2020 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor
Biotechnology and Biological Sciences Research Council
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/32768892
PII: S1367-5931(20)30094-6
Grant Number
BB/M011178/1
Subjects
Activity-based probes
Activity-based protein profiling
Chemical proteomics
Drug discovery
Reactivity-based protein profiling
Target identification and validation
Publication Status
Published online
Coverage Spatial
England
Date Publish Online
2020-08-05