GPRC6a is not required for the effects of a high-protein diet on body weight in mice
Author(s)
Type
Journal Article
Abstract
Objective: The G-protein coupled receptor family C group 6 member A (GPRC6A) is activated by
proteinogenic amino acids and may sense amino acids in the gastrointestinal tract and the brain. The
study investigated whether GPRC6A was necessary for the effects of low- and high-protein diets on
body weight and food intake in mice.
Methods: The role of GPRC6A in mediating the effects of a low-protein diet on body weight was investigated
in GPRC6a knockout (GPRC6a-KO) and wild-type (WT) mice fed a control diet (18% protein) or a
low-protein diet (6% protein) for 9 days. The role of GPRC6A in mediating the effects of a high-protein
diet on body weight was investigated in GPRC6a-KO and WT mice fed a control diet (18% protein) or a
high-protein diet (50% protein) for 5 weeks.
Results: A high-protein diet reduced body weight gain and food intake compared with a control diet in
both WT and GPRC6a-KO mice. A low-protein diet decreased body weight gain in GPRC6a-KO mice.
Conclusions: GPRC6A was not necessary for the effects of a low- or high-protein diet on body weight
and likely does not play a role in protein-induced satiety.
proteinogenic amino acids and may sense amino acids in the gastrointestinal tract and the brain. The
study investigated whether GPRC6A was necessary for the effects of low- and high-protein diets on
body weight and food intake in mice.
Methods: The role of GPRC6A in mediating the effects of a low-protein diet on body weight was investigated
in GPRC6a knockout (GPRC6a-KO) and wild-type (WT) mice fed a control diet (18% protein) or a
low-protein diet (6% protein) for 9 days. The role of GPRC6A in mediating the effects of a high-protein
diet on body weight was investigated in GPRC6a-KO and WT mice fed a control diet (18% protein) or a
high-protein diet (50% protein) for 5 weeks.
Results: A high-protein diet reduced body weight gain and food intake compared with a control diet in
both WT and GPRC6a-KO mice. A low-protein diet decreased body weight gain in GPRC6a-KO mice.
Conclusions: GPRC6A was not necessary for the effects of a low- or high-protein diet on body weight
and likely does not play a role in protein-induced satiety.
Date Issued
2015-06-01
Date Acceptance
2015-02-19
Citation
Obesity, 2015, 23 (6), pp.1194-1200
ISSN
1930-7381
Publisher
Wiley
Start Page
1194
End Page
1200
Journal / Book Title
Obesity
Volume
23
Issue
6
Copyright Statement
© 2015 The Obesity Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided
the original work is properly cited.
the original work is properly cited.
License URL
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Society for Endocrinology
NC3Rs (National Centre for the Replacement, Refinement and Reduction of Animals in Research)
Grant Number
BB/I001816/1
N/A
NC/K500379/1
Subjects
Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
Nutrition & Dietetics
AMINO ACID RECEPTOR
COUPLED RECEPTOR
FOOD-INTAKE
FAT DEPOSITION
KNOCKOUT MICE
L-ARGININE
CELLS
RATS
SECRETION
GUT
Publication Status
Published