Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer.
File(s)
Accepted version
OA Location
Author(s)
Lomax-Browne, Hannah J
Robertson, Claire
Antonopoulos, Aristotelis
Leathem, Anthony JC
Haslam, Stuart M
Type
Journal Article
Abstract
The lectin Helix pomatia agglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim. Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases. In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed. Detailed mass spectrometric structural analysis showed an increase in disialo-biantennary N-linked glycans on IgA1 from BCa patients (p < 0.0001: non-core fucosylated; p = 0.0345: core fucosylated) and increased asialo-Thomsen-Friedenreich antigen (TF) and disialo-TF antigens in the O-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals. An increase in Sambucus nigra binding was observed, suggestive of increased α2,6-linked sialic acid on IgA1 in BCa. Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ2 13.359; n = 114; p = 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively. Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue. This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication.
Date Issued
2019-04-06
Date Acceptance
2019-01-03
Citation
Interface Focus, 2019, 9 (2), pp.20180079-20180079
ISSN
2042-8898
Start Page
20180079
End Page
20180079
Journal / Book Title
Interface Focus
Volume
9
Issue
2
Copyright Statement
©2019 The Author(s) Published by the Royal Society. All rights reserved.
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/30842877
PII: rsfs20180079
Grant Number
BB/F008309/1
BB/K016164/1
Subjects
ELISA
IgA
breast cancer
glycosylation
lectin
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2019-02-15