Fluorescent quantum dots based lateral flow assay for rapid quantitative detection of ciliary neurotrophic factor in glaucoma
Author(s)
Type
Journal Article
Abstract
Early and accurate diagnosis of glaucoma is crucial to prevent the progressive deterioration that leads to irreversible vision loss. It is imperative to develop an effective screening tool for glaucoma. Ciliary neurotrophic factor (CNTF) is a tear biomarker implicated in glaucoma pathogenesis. Lateral flow assay (LFA) provides an ideal platform for detection of glaucoma in tear fluid. A quantum dot-based fluorescence LFA, integrated with a 3D printed readout box, is developed for fast (30 min), sensitive, and quantitative CNTF detection in tears. A standard curve is firstly generated for the quantitative detection of CNTF. The limit of detection (LOD) of the obtained LFA strip at 6.45 pg·mL−1 is comparable to that of the enzyme-linked immunosorbent assay (ELISA) at 6.42 pg·mL−1. This enables the identification of low CNTF levels (25.7 ± 14.9 pg·mL−1) reported in tear fluid from glaucoma patients. This LFA is found to be highly selective for CNTF and maintained consistent results in different pH condition. The strip remaines stablewhen stored in the darkat room temperature. A smartphone app is developed to simplify analysis and enable prompt and easily obtainable results. This method shows great potential to be a powerful tool for point-of-care glaucoma screening.
Date Issued
2024-08-21
Date Acceptance
2024-05-01
Citation
Advanced Materials Technologies, 2024, 9 (16)
ISSN
2365-709X
Publisher
Wiley
Journal / Book Title
Advanced Materials Technologies
Volume
9
Issue
16
Copyright Statement
© 2024 The Authors. Advanced Materials Technologies published byWiley-VCH GmbH. This is an open access article under the terms of theCreative Commons Attribution License, which permits use, distributionand reproduction in any medium, provided the original work is properlycited
License URL
Subjects
BIOMARKERS
ciliary neurotrophic factor
ELISA
fluorescence immunoassay
GANGLION-CELL SURVIVAL
glaucoma screening
GOLD
lateral flow assay
Materials Science
Materials Science, Multidisciplinary
OPEN-ANGLE GLAUCOMA
PH
quantum dot
Science & Technology
smartphone readout
TEARS
Technology
YIELD
Publication Status
Published
Article Number
2400238
Date Publish Online
2024-05-16