CRISPR-based biosensors for pathogenic biosafety
File(s)
Author(s)
Yang, Hao
Ledesma-Amaro, Rodrigo
Gao, Hong
Ren, Yao
Deng, Ruijie
Type
Journal Article
Abstract
Pathogenic biosafety is a worldwide concern. Tools for analyzing pathogenic biosafety, that are precise, rapid and field-deployable, are highly demanded. Recently developed biotechnological tools, especially those utilizing CRISPR/Cas systems which can couple with nanotechnologies, have enormous potential to achieve point-of-care (POC) testing for pathogen infection. In this review, we first introduce the working principle of class II CRISPR/Cas system for detecting nucleic acid and non-nucleic acid biomarkers, and highlight the molecular assays that leverage CRISPR technologies for POC detection. We summarize the application of CRISPR tools in detecting pathogens, including pathogenic bacteria, viruses, fungi and parasites and their variants, and highlight the profiling of pathogens’ genotypes or phenotypes, such as the viability, and drug-resistance. In addition, we discuss the challenges and opportunities of CRISPR-based biosensors in pathogenic biosafety analysis.
Date Issued
2023-05-15
Date Acceptance
2023-03-01
Citation
Biosensors and Bioelectronics, 2023, 228
ISSN
0956-5663
Publisher
Elsevier
Journal / Book Title
Biosensors and Bioelectronics
Volume
228
Copyright Statement
Copyright © Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/36893718
PII: S0956-5663(23)00131-8
Subjects
AMPLIFIED DETECTION
Biophysics
Biotechnology & Applied Microbiology
Chemistry
Chemistry, Analytical
CRISPR-CAS12A
DNA
Drug-resistance
Electrochemistry
FOOD
Fungi
IDENTIFICATION
ISOTHERMAL AMPLIFICATION
Life Sciences & Biomedicine
Nanoscience & Nanotechnology
NUCLEIC-ACID DETECTION
Parasites
Physical Sciences
PLATFORM
Point-of-care
SARS-COV-2
SARS-CoV-2 variants
Science & Technology
Science & Technology - Other Topics
SENSITIVE DETECTION
Viable bacteria
Publication Status
Published
Coverage Spatial
England
Article Number
115189
Date Publish Online
2023-03-02