The Andes have experienced an unprecedented wave of amphibian declines and extinctions that
are linked to a combination of habitat reduction and the spread of the fungal pathogen, Batrachochytrium
dendrobatidis (Bd). In the present study, a range of high-altitude habitats in Southern Ecuador were surveyed
for the presence of Bd. With a particular focus on Yacuri National Park, infection data are presented from
across the resident amphibian community. This community contains a once putatively extinct species which
was rediscovered in 2016, the Podocarpus Stubfoot Toad (Atelopus podocarpus). Across species, local Bd
prevalence was 73% in tadpoles (n = 41 individuals from three species) and 14% in adults (n = 43 individuals
from 14 species). Strikingly, 93% (14/15) of tested tadpoles of the recently described local endemic, Gastrotheca
yacuri, were infected with a high pathogen load, suggesting that this species likely acts as a reservoir of
infection in Yacuri. These fndings show that the threat of disease for A. podocarpus still exists, and that this
species requires urgent action to ensure its survival.