Novel methods in adrenal research: a metabolomics approach
Author(s)
Type
Journal Article
Abstract
Metabolic alterations have implications in a spectrum of tissue functions and disease. Aided by novel molecular biological and computational tools, our understanding of physiological and pathological processes underpinning endocrine and endocrine-related disease has significantly expanded over the last decade. Herein, we focus on novel metabolomics-related methodologies in adrenal research: in situ metabolomics by mass spectrometry imaging, steroid metabolomics by gas and liquid chromatography–mass spectrometry, energy pathway metabologenomics by liquid chromatography–mass spectrometry-based metabolomics of Krebs cycle intermediates, and cellular reprogramming to generate functional steroidogenic cells and hence to modulate the steroid metabolome. All four techniques to assess and/or modulate the metabolome in biological systems provide tremendous opportunities to manage neoplastic and non-neoplastic disease of the adrenal glands in the era of precision medicine. In this context, we discuss emerging clinical applications and/or promising metabolic-driven research towards diagnostic, prognostic, predictive and therapeutic biomarkers in tumours arising from the adrenal gland and extra-adrenal paraganglia as well as modern approaches to delineate and reprogram adrenal metabolism.
Date Issued
2019-03-05
Date Acceptance
2019-02-01
Citation
Histochemistry and Cell Biology, 2019, 151 (3), pp.201-216
ISSN
0948-6143
Publisher
Springer
Start Page
201
End Page
216
Journal / Book Title
Histochemistry and Cell Biology
Volume
151
Issue
3
Copyright Statement
© Springer-Verlag GmbH Germany, part of Springer Nature 2019
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/30725173
PII: 10.1007/s00418-019-01772-w
Subjects
ADDISONS-DISEASE
Adrenal glands
Cell Biology
Cellular reprogramming
CUSHINGS-SYNDROME
DEHYDROGENASE-ACTIVITY
DIABETES-MELLITUS
EMBRYONIC STEM-CELLS
In situ metabolomics
Life Sciences & Biomedicine
LIQUID-CHROMATOGRAPHY
MASS-SPECTROMETRY
Metabologenomics
Microscopy
MINERALOCORTICOID EXCESS
MUTANT P450 OXIDOREDUCTASE
PRIMARY ALDOSTERONISM
Science & Technology
Steroid metabolomics
Technology
Publication Status
Published
Coverage Spatial
Germany
Date Publish Online
2019-02-06