The high biodiversity of soil ecosystems is often attributed to their spatial heterogeneity at multiple scales, but studies on the small-scale spatial distribution of soil macrofauna are rare. This case study of an Ash – Field Maple woodland partially converted to conifer plantation investigates differences between species assemblages of soil and litter invertebrates, and plants, using multivariate ordination and indicator species analysis for eleven microhabitats.

Microhabitats representing the main body of uniform litter were compared with more localised microhabitats including dead wood and areas of wet soil. Species accumulation curves suggest that for this site it is more efficient to sample from varied microhabitats of limited spatial scale rather than the broad habitat areas when generating a species inventory. For comparative work sampling the main body of uniform litter is more appropriate, given that microhabitats vary from woodland to woodland and would make standardisation problematic.

Vegetation showed more distinctive microhabitat-specific species assemblages than soil and leaf litter invertebrates and was strongly associated with environmental variables. Microhabitats with distinct assemblages included dead wood habitats, which had a high proportion of saproxylic species; a highly disturbed microhabitat with distinct plant and soil species characteristic of ruderal habitats and seeps with earthworm species rarely sampled in standard soil biodiversity surveys.

The leaf litter in the conifer plantation area was species poor and the biodiversity quantified was considerably enhanced by the sampling from the additional microhabitats - illustrating the importance of small-scale heterogeneity for increasing plant and soil macrofauna biodiversity at this site.