A new line of research on system and signals design for Wireless Information and Power Transfer (WIPT) has recently emerged. Various system architectures and signal design strategies are proposed based on various different modelling of the energy receivers. To a great extent, the study of those strategies and architectures has so far been limited to theoretical performance analysis. In this thesis, we study the real over-the-air performance of the system architectures and signal strategies for WIPT. To that end, we have designed, prototyped and experimented an innovative WIPT system combined with Wireless Power Transfer (WPT) via RF and Wireless Information Transfer (WIT) based on Software-Defined Radio (SDR) that can operate in open-loop and closed-loop (with channel acquisition at the transmitter) modes. The prototype consists of important building blocks, namely the energy transmitter (ET), information transmitter and receiver (IT/R), channel estimator (CE), Feedback Transmitter and Receiver (FT/R), and the energy harvester(EH). First, WPT signal strategies designed based on different energy receiver models were evaluated in a real wireless environment using a prototype. The experiments have been conducted in a variety of deployments, including frequency flat and frequency selective channels, under static and mobility conditions. Experiments highlight that a channel-adaptive WPT signal strategy based on non-linear diode model outperforms other strategies. Second, feedback architectures for acquiring channel state information (CSI) are considered and present each architecture’s strength and the possibility of improvement using experimental results. Third, SWIPT signals strategies are evaluated in both aspects of energy harvesting and information throughput and investigated to minimise the tradeoff between WPT and WIT performance. Two different SWIPT receiver architectures, namely time-switching and power-splitting, are used and examined its performance. Last but not least, a new modulation for SWIPT with EH and information decoder (ID) integrated receiver architecture is proposed based on the observations from the real-world experiments. Overall, the proposed signal strategies for WPT and WIPT is evaluated by analytical models, simulations, and experiment in the real-world setup and the results are compared between different strategies. The experiments also confirm the crucial and beneficial role played by the energy harvester nonlinearity, frequency diversity, and spatial diversity and represent the gains from different aspects can be cumulative. Observations acquired in practical and realistic experiments accelerate a motivation to design a novel signal strategy and give an insight into how the signal design factors are played a role.