Glycoengineering Chinese hamster ovary cells: a short history
File(s)
Published version
Author(s)
Donini, roberto
Haslam, stuart
Kontoravdi, Kleio
Type
Journal Article
Abstract
Biotherapeutic glycoproteins have revolutionised the field of pharmaceuticals, with new discoveries and continuous improvements underpinning the rapid growth of this industry. N-glycosylation is a critical quality attribute of biotherapeutic glycoproteins that influences the efficacy, half-life and immunogenicity of these drugs. This review will focus on the advances and future directions of remodelling N-glycosylation in Chinese hamster ovary (CHO) cells, which are the workhorse of recombinant biotherapeutic production, with particular emphasis on antibody products, using strategies such as cell line and protein backbone engineering.
Date Issued
2021-04-01
Date Acceptance
2021-02-14
Citation
Biochemical Society Transactions, 2021, 49 (2), pp.915-931
ISSN
0300-5127
Publisher
Portland Press
Start Page
915
End Page
931
Journal / Book Title
Biochemical Society Transactions
Volume
49
Issue
2
Copyright Statement
© 2021 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under theCreative Commons Attribution License 4.0 (https://creativecommons.org/licenses/by/4.0/)
License URL
Subjects
cell engineering
erythropoietin
glycomics
glycoproteomics
glycosylation
monoclonal antibodies
0601 Biochemistry and Cell Biology
1101 Medical Biochemistry and Metabolomics
Biochemistry & Molecular Biology
Publication Status
Published
Date Publish Online
2021-03-11