jULIEs: nanostructured polytrodes for low traumatic extracellular recordings and stimulation in the mammalian brain
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Objective. Extracellular microelectrode techniques are the most widely used approach to interrogate neuronal populations. However, regardless of the manufacturing method used, damage to the vasculature and circuit function during probe insertion remains a concern. This issue can be mitigated by minimising the footprint of the probe used. Reducing the size of probes typically requires either a reduction in the number of channels present in the probe, or a reduction in the individual channel area. Both lead to less effective coupling between the probe and extracellular signals of interest. Approach. Here, we show that continuously drawn SiO2-insulated ultra-microelectrode fibres offer an attractive substrate to address these challenges. Individual fibres can be fabricated to >10 m continuous stretches and a selection of diameters below 30 µm with low resistance (<100 Ω mm−1) continuously conductive metal core of <10 µm and atomically flat smooth shank surfaces. To optimize the properties of the miniaturised electrode-tissue interface, we electrodeposit rough Au structures followed by ∼20 nm IrOx film resulting in the reduction of the interfacial impedance to <500 kΩ at 1 kHz. Main results. We demonstrate that these ultra-low impedance electrodes can record and stimulate both single and multi-unit activity with minimal tissue disturbance and exceptional signal-to-noise ratio in both superficial (∼40 µm) and deep (∼6 mm) structures of the mouse brain. Further, we show that sensor modifications are stable and probe manufacturing is reproducible. Significance. Minimally perturbing bidirectional neural interfacing can reveal circuit function in the mammalian brain in vivo.
Date Issued
2022-02-01
Date Acceptance
2022-09-02
Citation
Journal of Neural Engineering, 2022, 19 (1)
ISSN
1741-2552
Publisher
IOP Publishing
Journal / Book Title
Journal of Neural Engineering
Volume
19
Issue
1
Copyright Statement
© 2022 The Author(s). Published by IOP Publishing Ltd. Original content from this work may be used under the terms of the Creative Commons Attribution 4.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000762053700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
CHANNELS
DEPENDENCE
ELECTRODES
Engineering
Engineering, Biomedical
extracellular
HIGH-DENSITY
in vivo
IRIDIUM OXIDE-FILMS
LARGE-SCALE
Life Sciences & Biomedicine
LOCAL CIRCUITS
minimally perturbing
nanostructured
Neurosciences
Neurosciences & Neurology
polytrodes
RESPONSES
scalable
Science & Technology
SILICON PROBES
Technology
Publication Status
Published
Coverage Spatial
England
Article Number
ARTN 016041
Date Publish Online
2022-02-28