Derivatization to reduce background interferences for simultaneous quantitation of trimethylamine (TMA) and trimethylamine-N-oxide (TMAO) using liquid chromatography with tandem mass spectrometry
File(s)
Published version
Author(s)
Valdivia Garcia, Maria
Bi, Yang
Abaakil, Kaoutar
Li, Jia
Type
Journal Article
Abstract
Trimethylamine (TMA) and trimethylamine-N-oxide (TMAO) play a crucial role in many biochemical processes within diverse organisms including animals, plants, fungi and bacteria. Studies have linked these metabolites with cardiovascular and kidney diseases; however, emerging evidence demonstrates their protective properties. Owing to these controversies and co-existence of these metabolites in biological samples, it is crucial to accurately quantify these metabolites to associate their concentrations with various physiological and pathophysiological conditions to elucidate their potential roles. We reported interferences on TMA quantification without derivatizing the analyte. A combined sample preparation method, including sample derivatization with ethyl bromoacetate and use of ion pairing reagent (sodium heptane sulfonate), minimized these interferences and provided improved accuracy and precision for simultaneous quantification of TMA and TMAO. The linearity for TMAO ranged from 0.01 µM to 300 µM and 0.1 µM - 300 µM for TMA. With the application of this method, we reported that the circulating concentrations of TMA was 4 times higher in male mice (33.1 ± 5.9 µmol/L) compared to females (8.3 ± 1.39 µmol/L), whereas TMAO levels were 6 times lower in male (7.2 ± 0.4 µmol/L) than female mice (42.1 ± 4.5 µmol/L). In contrast, concentrations of TMA and TMAO in the colonic tissue did not differ significantly between males and females. The robust analytical method for simultaneously quantify TMA and TMAO presents a significant value in facilitating investigations on TMA and TMAO biology.
Date Issued
2025-01-01
Date Acceptance
2024-09-16
Citation
Journal of Pharmaceutical and Biomedical Analysis, 2025, 252
ISSN
0731-7085
Publisher
Elsevier
Journal / Book Title
Journal of Pharmaceutical and Biomedical Analysis
Volume
252
Copyright Statement
© 2024 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
Identifier
https://www.sciencedirect.com/science/article/pii/S0731708524005223
Publication Status
Published
Article Number
116480
Date Publish Online
2024-09-20