Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors
File(s)
Published version
Author(s)
Type
Conference Paper
Abstract
Molecular rotors are fluorophores that have a fluorescence quantum yield that depends upon intermolecular rotation. The fluorescence quantum yield, intensity and lifetime of molecular rotors all vary as functions of viscosity, as high viscosities inhibit intermolecular rotation and cause an increase in the non-radiative decay rate. As such, molecular rotors can be used to probe viscosity on microscopic scales. Here, we apply fluorescence lifetime imaging microscopy (FLIM) to measure the fluorescence lifetimes of three different molecular rotors, in order to determine the microscopic viscosity in two model systems with significant biological interest. First, the constituents of a novel protocell – a model of a prebiotic cell – were studied using the molecular rotors BODIPY C10 and kiton red. Second, amyloid formation was investigated using the molecular rotor Cy3. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Editor(s)
Farkas, DL
Nicolau, DV
Leif, RC
Date Issued
2014-03-04
Date Acceptance
2014-02-03
Citation
maging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 2014, 8947
ISSN
0277-786X
Publisher
SPIE- Society of Photo-optical Instrumentation Engineers
Journal / Book Title
maging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Volume
8947
Copyright Statement
© 2014 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000336037200028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Source
Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Subjects
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Cell & Tissue Engineering
Optics
Radiology, Nuclear Medicine & Medical Imaging
Cell Biology
molecular rotor
fluorescence
fluorescence lifetime
FLIM
viscosity
confocal microscopy
protocells
protein aggregation
amyloid
lysozyme
neurodegeneration
LIVE CELLS
VISCOSITY
AGGREGATION
Publication Status
Published
Start Date
2014-02-03
Finish Date
2014-02-06
Coverage Spatial
San Francisco, CA