Production of selenium nanoparticles occurs through
an interconnected pathway of sulphur metabolism and
oxidative stress response in Pseudomonas putida KT2440
an interconnected pathway of sulphur metabolism and
oxidative stress response in Pseudomonas putida KT2440
Author(s)
Type
Journal Article
Abstract
The soil bacterium Pseudomonas putida KT2440 has been shown to produce selenium nanoparticles aerobically from selenite; however, the molecular actors involved in this process are unknown. Here, through a combination of genetic and analytical techniques, we report the first insights into selenite metabolism in this bacterium. Our results suggest that the reduction of selenite occurs through an interconnected metabolic network involving central metabolic reactions, sulphur metabolism, and the response to oxidative stress. Genes such as sucA, D2HGDH and PP_3148 revealed that the 2-ketoglutarate and glutamate metabolism is important to convert selenite into selenium. On the other hand, mutations affecting the activity of the sulphite reductase decreased the bacteria's ability to transform selenite. Other genes related to sulphur metabolism (ssuEF, sfnCE, sqrR, sqr and pdo2) and stress response (gqr, lsfA, ahpCF and sadI) were also identified as involved in selenite transformation. Interestingly, suppression of genes sqrR, sqr and pdo2 resulted in the production of selenium nanoparticles at a higher rate than the wild-type strain, which is of biotechnological interest. The data provided in this study brings us closer to understanding the metabolism of selenium in bacteria and offers new targets for the development of biotechnological tools for the production of selenium nanoparticles.
Date Issued
2023-05
Date Acceptance
2022-12-22
Citation
Microbial Biotechnology, 2023, 16 (5), pp.931-946
ISSN
1751-7907
Publisher
Wiley
Start Page
931
End Page
946
Journal / Book Title
Microbial Biotechnology
Volume
16
Issue
5
Copyright Statement
© 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000919067800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
AERUGINOSA
Biotechnology & Applied Microbiology
CYTOCHROME-C BIOGENESIS
ENTNER-DOUDOROFF
ESCHERICHIA-COLI
GLUTATHIONE
HYDROPEROXIDE REDUCTASE AHPCF
IDENTIFICATION
Life Sciences & Biomedicine
Microbiology
RHODOSPIRILLUM-RUBRUM
Science & Technology
THAUERA-SELENATIS
TRANSPORTER
Publication Status
Published
Date Publish Online
2023-01-22