Comparative metabolomic sampling of upper and lower airways by four different methods to identify biochemicals that may support bacterial growth
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Bacteria need nutrients from the host environment to survive, yet we know little about which biochemicals are present in the airways (the metabolome), which of these biochemicals are essential for bacterial growth and how they change with airway disease. The aims of this pilot study were to develop and compare methodologies for sampling the upper and lower airway metabolomes and to identify biochemicals present in the airways that could potentially support bacterial growth. Eight healthy human volunteers were sampled by four methods: two standard approaches - nasal lavage and induced sputum, and two using a novel platform, synthetic adsorptive matrix (SAM) strips—nasosorption and bronchosorption. Collected samples were analyzed by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectroscopy (UPLC-MS/MS). Five hundred and eighty-one biochemicals were recovered from the airways belonging to a range of metabolomic super-pathways. We observed significant differences between the sampling approaches. Significantly more biochemicals were recovered when SAM strips were used, compared to standard sampling techniques. A range of biochemicals that could support bacterial growth were detected in the different samples. This work demonstrates for the first time that SAM strips are a highly effective method for sampling the airway metabolome. This work will assist further studies to understand how changes in the airway metabolome affect bacterial infection in patients with underlying airway disease.
Date Issued
2018-12-18
Date Acceptance
2018-11-30
Citation
Frontiers in Cellular and Infection Microbiology, 2018, 8
ISSN
2235-2988
Publisher
Frontiers Media
Journal / Book Title
Frontiers in Cellular and Infection Microbiology
Volume
8
Copyright Statement
© 2018 Farne, Groves, Gill, Stokes, McCulloch, Karoly, Trujillo-Torralbo, Johnston, Mallia and Tregoning. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY, https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Sponsor
Imperial College Healthcare NHS Trust- BRC Funding
Royal Brompton & Harefield NHS Foundation Trust
Imperial College Healthcare NHS Trust- BRC Funding
Grant Number
RDA02 79560
N/A
RDA02
Subjects
Science & Technology
Life Sciences & Biomedicine
Immunology
Microbiology
airway metabolome
airway sampling methods
healthy volunteers
upper airways
lower airways
synthetic adsorptive matrix strips
EXHALED BREATH CONDENSATE
INFLAMMATION
GLUCOSE
LOAD
EXACERBATIONS
TEMPERATURE
INCREASES
MEDIATORS
DISEASES
ASTHMA
Publication Status
Published
Article Number
432