Building Yarrowia lipolytica cell factories for advanced biomanufacturing: challenges and solutions
File(s)
Author(s)
Type
Journal Article
Abstract
Microbial cell factories have shown great potential for industrial production with the benefit of being environmentally friendly and sustainable. Yarrowia lipolytica is a promising and superior non-model host for biomanufacturing due to its cumulated advantages compared to model microorganisms, such as high fluxes of metabolic precursors (acetyl-CoA and malonyl-CoA) and its naturally hydrophobic microenvironment. However, although diverse compounds have been synthesized in Y. lipolytica cell factories, most of the relevant studies have not reached the level of industrialization and commercialization due to a number of remaining challenges, including unbalanced metabolic flux, conflict between cell growth and product synthesis, and cytotoxic effects. Here, various metabolic engineering strategies for solving the challenges are summarized, which is developing fast and extremely conducive to rational design and reconstruction of robust Y. lipolytica cell factories for advanced biomanufacturing. Finally, future engineering efforts for enhancing the production efficiency of this platform strain are highlighted.
Date Issued
2024-01-10
Date Acceptance
2023-12-06
Citation
Journal of Agricultural and Food Chemistry, 2024, 72 (1), pp.94-107
ISSN
0021-8561
Publisher
American Chemical Society
Start Page
94
End Page
107
Journal / Book Title
Journal of Agricultural and Food Chemistry
Volume
72
Issue
1
Copyright Statement
Copyright © 2023 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, after peer review and technical editing by the publisher. To access the final edited and published work see [https://doi.org/10.1021/acs.jafc.3c07889, see ACS Articles on Request https://pubs.acs.org/page/4authors/benefits/index.html#articles-request]
Identifier
https://pubs.acs.org/doi/10.1021/acs.jafc.3c07889
Subjects
ACID
advanced biomanufacturing
Agriculture
Agriculture, Multidisciplinary
BIOSENSORS
challenges
Chemistry
Chemistry, Applied
ESCHERICHIA-COLI
EXPRESSION
Food Science & Technology
HETEROLOGOUS BIOSYNTHESIS
Life Sciences & Biomedicine
microbial cell factories
ORGANELLES
PATHWAY
Physical Sciences
Science & Technology
STRATEGIES
synthetic biology
TERPENOIDS
Yarrowialipolytica
Publication Status
Published
Date Publish Online
2023-12-21