Small molecule electro-optical binding assay using nanopores
File(s)
Published version
Author(s)
Cai, shenglin
Sze, Yuk Ying Jasmine
Ivanov, Aleksandar
Edel, Joshua
Type
Journal Article
Abstract
The identification of short nucleic acids and proteins at the single molecule level is a major driving force for the development of novel detection strategies. Nanopore sensing has been gaining in prominence due to its label-free operation and single molecule sensitivity. However, it remains challenging to detect small molecules selectively. Here we propose to combine the electrical sensing modality of a nanopore with fluorescence-based detection. Selectivity is achieved by grafting either molecular beacons, complementary DNA, or proteins to a DNA molecular carrier. We show that the fraction of synchronised events between the electrical and optical channels, can be used to perform single molecule binding assays without the need to directly label the analyte. Such a strategy can be used to detect targets in complex biological fluids such as human serum and urine. Future optimisation of this technology may enable novel assays for quantitative protein detection as well as gene mutation analysis with applications in next-generation clinical sample analysis.
Date Issued
2019-04-17
Date Acceptance
2019-03-12
Citation
Nature Communications, 2019, 10, pp.1-9
ISSN
2041-1723
Publisher
Nature Research (part of Springer Nature)
Start Page
1
End Page
9
Journal / Book Title
Nature Communications
Volume
10
Copyright Statement
© 2019 The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
License URL
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Commission of the European Communities
Biotechnology and Biological Sciences Research Council (BBSRC)
Identifier
https://www.nature.com/articles/s41467-019-09476-4
Grant Number
EP/P011985/1
724300
BB/R022429/1
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SOLID-STATE NANOPORES
ELECTRONIC DETECTION
PROTEIN-DETECTION
APTAMER BEACONS
DNA
FLUORESCENCE
BIOMOLECULES
THROMBIN
Biosensing Techniques
DNA
Fluorescence
Humans
Nanopores
Nanotechnology
Proteins
Humans
Proteins
DNA
Biosensing Techniques
Nanotechnology
Fluorescence
Nanopores
Publication Status
Published
Article Number
1797 (2019)
Date Publish Online
2019-04-17