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The utility of ultra-high performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS) for clinically relevant steroid analysis
File(s)
Author(s)
Storbeck, Karl-Heinz
Gilligan, Lorna
Jenkinson, Carl
Baranowski, Elizabeth S
Quanson, Jonathan L
Type
Journal Article
Abstract
Liquid chromatography tandem mass spectrometry (LC-MS/MS) assays are considered the reference standard for serum steroid hormone analyses, while full urinary steroid profiles are only achievable by gas chromatography (GC–MS). Both LC-MS/MS and GC–MS have well documented strengths and limitations. Recently, commercial ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) systems have been developed. These systems combine the resolution of GC with the high-throughput capabilities of UHPLC. Uptake of this new technology into research and clinical labs has been slow, possibly due to the perceived increase in complexity. Here we therefore present fundamental principles of UHPSFC-MS/MS and the likely applications for this technology in the clinical research setting, while commenting on potential hurdles based on our experience to date.
Date Issued
2018-05-15
Date Acceptance
2018-03-16
Citation
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2018, 1085, pp.36-41
URI
URL
DOI
ISSN
1570-0232
Publisher
Elsevier
Start Page
36
End Page
41
Journal / Book Title
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume
1085
Copyright Statement
© 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/29627638
PII: S1570-0232(18)30116-8
Subjects
Biochemical Research Methods
Biochemistry & Molecular Biology
BIOLOGICAL SAMPLES
Chemistry
Chemistry, Analytical
DERIVATIZATION
Gas chromatography-mass spectrometry
GAS-CHROMATOGRAPHY
INTERFACE
IONIZATION
Life Sciences & Biomedicine
LIQUID-CHROMATOGRAPHY
METABOLISM
MS
Physical Sciences
POLYCYSTIC-OVARY-SYNDROME
Science & Technology
Steroid analysis
Ultra-high performance liquid chromatography-tandem mass spectrometry
Ultra-high performance supercritical fluid chromatography-tandem mass spectrometry
URINE
Publication Status
Published
Coverage Spatial
Netherlands
Date Publish Online
2018-03-20