Robotic automation of in vivo two photon targeted whole-cell patch clamp electrophysiology
File(s)
Published version
Accepted version
Author(s)
Type
Journal Article
Abstract
Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed “blind,” meaning that throughput for sampling some genetically or morphologically defined cell types is unacceptably low. One solution to this problem is to use two-photon microscopy to target fluorescently labeled neurons. Combining this with robotic automation is difficult, however, as micropipette penetration induces tissue deformation, moving target cells from their initial location. Here we describe a platform for automated two-photon targeted patch-clamp recording, which solves this problem by making use of a closed loop visual servo algorithm. Our system keeps the target cell in focus while iteratively adjusting the pipette approach trajectory to compensate for tissue motion. We demonstrate platform validation with patch-clamp recordings from a variety of cells in the mouse neocortex and cerebellum.
Date Issued
2017-08-30
Date Acceptance
2017-08-12
Citation
Neuron, 2017, 95 (5), pp.1048-1055.e3
ISSN
0896-6273
Publisher
Elsevier
Start Page
1048
End Page
1055.e3
Journal / Book Title
Neuron
Volume
95
Issue
5
Copyright Statement
© 2017 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Commission of the European Communities
Scientifica Ltd
Medical Research Council (MRC)
Identifier
https://www.sciencedirect.com/science/article/pii/S089662731730733X?via%3Dihub
Grant Number
BB/K001817/1
289146
BB/K001871/1
G1000512
Subjects
Science & Technology
Life Sciences & Biomedicine
Neurosciences
Neurosciences & Neurology
MAMMALIAN BRAIN
NEURONS
RECORDINGS
NETWORKS
SLICES
CORTEX
automated neurophysiology
multiphoton microscopy
patch clamp
robotic automation
Animals
Automation
Cerebellum
Mice
Neocortex
Neurons
Patch-Clamp Techniques
Photons
Robotics
Cerebellum
Neocortex
Neurons
Animals
Mice
Patch-Clamp Techniques
Robotics
Photons
Automation
1109 Neurosciences
1701 Psychology
1702 Cognitive Sciences
Neurology & Neurosurgery
Publication Status
Published
Date Publish Online
2017-08-30