Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader
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Published version
Author(s)
Type
Journal Article
Abstract
Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.
Date Issued
2019-02-20
Date Acceptance
2019-01-28
Citation
Nature Communications, 2019, 10, pp.1-10
ISSN
2041-1723
Publisher
Nature Research (part of Springer Nature)
Start Page
1
End Page
10
Journal / Book Title
Nature Communications
Volume
10
Copyright Statement
© 2019 The Author(s). This article is licensed under a Creative CommonsAttribution 4.0 International License, which permits use, sharing,adaptation, distribution and reproduction in any medium or format, as long as you giveappropriate credit to the original author(s) and the source, provide a link to the CreativeCommons license, and indicate if changes were made. The images or other third partymaterial in this article are included in the article’s Creative Commons license, unlessindicated otherwise in a credit line to the material. If material is not included in thearticle’s Creative Commons license and your intended use is not permitted by statutoryregulation or exceeds the permitted use, you will need to obtain permission directly fromthe copyright holder. To view a copy of this license, visithttp://creativecommons.org/licenses/by/4.0/.
Identifier
https://www.nature.com/articles/s41467-019-08799-6
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SENSORS
PERFORMANCE
BIOSENSORS
SYSTEM
Publication Status
Published
Article Number
ARTN 852
Date Publish Online
2019-02-20