pCORE, a set of open modular plasmids compatible with golden gate assembly
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Published version (article in press)
Author(s)
Type
Journal Article
Abstract
Modular DNA assembly based Golden Gate cloning (MoClo) is a widely used framework to assemble genetic constructs. A number of MoClo “destination vectors” have been developed, however, available options are still limited with regards to copy number and antibiotic resistance in bacteria, vector compactness, and industry researchers’ access to materials. Here, we assembled combinations of five resistance cassettes, four origins of replication and two colour markers to develop a collection of compact MoClo-compatible pCORE destination vectors. pCORE vectors support MoClo Level 1 and Level 2 assembly, and can serve as starting points for developing vectors for other hosts. We tested the performance of our vector collection in Escherichia coli, Agrobacterium tumefaciens and Saccharomyces cerevisiae. pCORE vectors are available to academia and industry under the terms of OpenMTA licence.
Date Acceptance
2026-08-07
Citation
Scientific Reports
ISSN
2045-2322
Publisher
Nature Portfolio
Journal / Book Title
Scientific Reports
Copyright Statement
© The Author(s) 2026. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
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Publication Status
Published online
Date Publish Online
2026-08-15