Synthetic developmental biology: molecular tools to re-design plant shoots and roots
File(s)
Accepted version
Author(s)
Kocaoglan, Elif Gediz
Radhakrishnan, Dhanya
Nakayama, Naomi
Type
Journal Article
Abstract
Plant morphology and anatomy strongly influence agricultural yield. Crop domestication has strived for desirable growth and developmental traits, such as larger and more fruits and semi-dwarf architecture. Genetic engineering accelerated rational, purpose-driven engineering of plant development, but it can be unpredictable - subtle or pleiotropic in the resulting effect. Developmental pathways are complex and riddled with environmental and hormonal inputs, as well as feedback and feedforward interactions, which occur at specific times and places in a growing multicellular organism. Rational modification of plant development would likely benefit from precision engineering based on synthetic biology approaches. This review outlines recently developed synthetic biology technologies for plant systems and highlights their potential for engineering plant growth and development. Streamlined and high-capacity genetic construction methods (Golden Gate DNA Assembly frameworks and toolkits) allow fast and variation-series cloning of multigene transgene constructs. This, together with the suite of gene regulation tools (e.g., cell-type specific promoters, logic gates, and multiplex regulation systems), is starting to enable developmental pathway engineering with predictable outcomes in model plant and crop species.
Date Issued
2023-07-18
Date Acceptance
2023-05-04
Citation
Journal of Experimental Botany, 2023, 74 (13), pp.3864-3876
ISSN
0022-0957
Publisher
Oxford University Press
Start Page
3864
End Page
3876
Journal / Book Title
Journal of Experimental Botany
Volume
74
Issue
13
Copyright Statement
Copyright © 2023 Oxford University Press. This is a pre-copy-editing, author-produced version of an article accepted for publication in Journal of Experimental Botany following peer review. The definitive publisher-authenticated version Elif Gediz Kocaoglan and others, Synthetic developmental biology: Molecular tools to re-design plant shoots and roots, Journal of Experimental Botany, 2023;, erad169 is available online at: https://doi.org/10.1093/jxb/erad169
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/37155965
PII: 7157064
Subjects
growth and development
Phytobricks
Plant synthetic biology
precision breeding
synthetic developmental biology
synthetic morphology
Publication Status
Published
Coverage Spatial
England
Article Number
erad169
Date Publish Online
2023-05-08