Long term effects of bioactive glass particulates on dental pulp stem cells in vitro
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Bioactive glasses (BG) are known for their ability to induce bone formation by the action of their dissolution products. Glasses can deliver active ions at a sustained rate, determined by their composition and surface area. Nanoporous sol-gel derived BGs can biodegrade rapidly, which can lead to a detrimental burst release of ions and a pH rise. The addition of phosphate into the glass can buffer the pH during dissolution. Here, dissolution of BG with composition 60 mol% SiO2, 28 mol% CaO and 12 mol% P2O5 at 600 g/ml were investigated. Initially, the dissolution and apatite formation of the BG particulates were examined in simulated body fluid using FTIR and XRD. BG particulates were indirectly exposed to dental pulp stem cells, and the effect of 14 days continuous ion release on human dental pulp stem cells (hDPSC) viability and differentiation was evaluated. Alamar blue assay showed that cell proliferation was not inhibited by the continuous release of Ca, P and soluble silica. In fact, hDPSC in the presence of BG particulate displayed a higher density of mineralized nodules than untreated cells, as assessed by Alizarin red. The results will have a great contribution to the in vivo application of this particular BG.
Date Issued
2017-12-07
Date Acceptance
2017-11-18
Citation
Biomedical Glasses, 2017, 3 (1), pp.96-103
ISSN
2299-3932
Publisher
Walter de Gruyter GmbH
Start Page
96
End Page
103
Journal / Book Title
Biomedical Glasses
Volume
3
Issue
1
Copyright Statement
©2017 S. Gholamiet al., published by De Gruyter Open.This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/)
Publication Status
Published
Date Publish Online
2017-12-07