Quantitative brain-derived neurotrophic factor lateral flow assay for point-of-care detection of glaucoma
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Glaucoma, a ruinous group of eye diseases with progressive degeneration of the optic nerve and vision loss, is the leading cause of irreversible blindness. Accurate and timely diagnosis of glaucoma is critical to promote secondary prevention and early disease-modifying therapies. Reliable, cheap, and rapid tests for measuring disease activities are highly required. Brain-derived neurotrophic factor (BDNF) plays an important role in maintaining the function and survival of the central nervous system. Decreased BDNF levels in tear fluid can be seen in glaucoma patients, which indicates that BDNF can be regarded as a novel biomarker for glaucoma. Conventional ELISA is the standard method to measure the BDNF level, but the multi-step operation and strict storage conditions limit its usage in point-of-care settings. Herein, a one-step and a portable glaucoma detection method was developed based on the lateral flow assay (LFA) to quantify the BDNF concentration in artificial tear fluids. The results of the LFA were analyzed by using a portable and low-cost system consisting of a smartphone camera and a dark readout box fabricated by 3D printing. The concentration of BDNF was quantified by analyzing the colorimetric intensity of the test line and the control line. This assay yields reliable quantitative results from 25 to 300 pg mL-1 with an experimental detection limit of 14.12 pg mL-1. The LFA shows a high selectivity for BDNF and high stability in different pH environments. It can be readily adapted for sensitive and quantitative testing of BDNF in a point-of-care setting. The BDNF LFA strip shows it has great potential to be used in early glaucoma detection.
Date Issued
2022-08-18
Date Acceptance
2022-08-02
Citation
Lab on a Chip: miniaturisation for chemistry, physics, biology, materials science and bioengineering, 2022, 22 (18), pp.3521-3532
ISSN
1473-0189
Publisher
Royal Society of Chemistry
Start Page
3521
End Page
3532
Journal / Book Title
Lab on a Chip: miniaturisation for chemistry, physics, biology, materials science and bioengineering
Volume
22
Issue
18
Copyright Statement
© The Royal Society of Chemistry 2022. his article is licensed under aCreative Commons Attribution 3.0 Unported Licence (https://creativecommons.org/licenses/by/3.0/)
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/35979801
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2022-08-02