The production of ethanol fuel involves separating water and ethanol by distillation and dehydration in an energy intensive process. To reduce the carbon footprint, hydrous ethanol is seen as a promising alternative. This study focused on the effect of water content in ethanol on flame development in a single-cylinder optical spark-ignition engine. Ethanol with 0%, 4% and 10% water content per volume was used with Port Fuel Injection (PFI) and Direct Injection (DI) for homogeneous and heterogeneous mixture preparation. Tests were also conducted with iso-octane for comparison. Flames captured by Planar Laser Induced Fluorescence (PLIF) of OH were spatially filtered to remove scales larger than the flame size, then the flame brush was quantified in terms of thickness, crossing frequency between instantaneous and filtered contours, and curvature. Increasing water content resulted in slower flame growth, with 10% hydrous exhibiting marginally higher flame speed than iso-octane. PFI led to faster flame growth and thicker flame brushes than DI at the same timing after ignition. When the brush thickness was normalised by flame radius, all hydrous fuels and iso-octane exhibited similar values with PFI, whilst anhydrous ethanol exhibited the thickest normalised brush. The alcohols maintained similar normalised thicknesses with DI and iso-octane exhibited the thickest normalised brush. When flames of the same radii were considered with PFI at the same timing, the fuels with sequentially lower laminar burning velocity were associated with larger crossing frequency. For PFI, increasing water content resulted in decreased flame roundness irrespective of size, with the 4% hydrous and iso-octane exhibiting similar values. With DI, anhydrous ethanol flames were rounder than the 4% hydrous when averaged over all cycles, but marginally rounder than the 10% hydrous and iso-octane. Increasing water content widened the curvature distribution and increased the mean with PFI. Flame roundness decreased with increase in mean curvature and Lewis number for both PFI and DI.