In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification
File(s)
Published version
Author(s)
O'Connor, MJ
Bishop, K
Workman, RG
Maddison, BC
Gough, KC
Type
Journal Article
Abstract
The in vitro amplification of prions by serial protein misfolding cyclic amplification has been shown to detect PrPSc to levels at least as sensitive as rodent bioassay but in a fraction of the time. Bovine spongiform encephalopathy is a zoonotic prion disease in cattle and has been shown to occur in 3 distinct forms, classical BSE (C-BSE) and 2 atypical BSE forms (L-BSE and H-BSE). Atypical forms are usually detected in asymptomatic, older cattle and are suggested to be spontaneous forms of the disease. Here, we show the development of a serial protein misfolding cyclic amplification method for the detection of H-BSE. The assay could detect PrPSc from 3 distinct experimental isolates of H-BSE, could detect PrPSc in as little as 1×10−12 g of brain material and was highly specific. Additionally, the product of serial protein misfolding cyclic amplification at all dilutions of seed analyzed could be readily distinguished from L-BSE, which did not amplify, and C-BSE, which had PrPSc with distinct protease K-resistance and protease K-resistant PrPSc molecular weights.
Date Issued
2017-02-08
Date Acceptance
2016-11-04
Citation
Prion, 2017, 11 (1), pp.54-64
ISSN
1933-6896
Publisher
Taylor & Francis
Start Page
54
End Page
64
Journal / Book Title
Prion
Volume
11
Issue
1
Copyright Statement
© 2017 Matthew J. O’Connor, Keith Bishop, Robert G. Workman, Ben C. Maddison, and Kevin C. Gough.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-
Commercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits
non-commercial re-use, distribution, and reproduction in any medium, provided the original work is prop-
erly cited, and is not altered, transformed, or built upon in any way.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-
Commercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits
non-commercial re-use, distribution, and reproduction in any medium, provided the original work is prop-
erly cited, and is not altered, transformed, or built upon in any way.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000396024200007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
atypical prions
bovine spongiform encephalopathy
H-type BSE
PMCA
CREUTZFELDT-JAKOB-DISEASE
PRION DISEASES
BSE
DISCRIMINATION
TRANSMISSION
CONVERSION
PRPSC
RISK
Publication Status
Published