Fibroblastic reticular cells provide a supportive niche for lymph node-resident macrophages
Author(s)
D'Rozario, Joshua
Knoblich, Konstantin
Luetge, Mechthild
Shibayama, Christian Perez
Cheng, Hung-Wei
Type
Journal Article
Abstract
The lymph node (LN) is home to resident macrophage populations that are essential for immune function and homeostasis, but key factors controlling this niche are undefined. Here, we show that fibroblastic reticular cells (FRCs) are an essential component of the LN macrophage niche. Genetic ablation of FRCs caused rapid loss of macrophages and monocytes from LNs across two in vivo models. Macrophages co-localized with FRCs in human LNs, and murine single-cell RNA-sequencing revealed that FRC subsets broadly expressed master macrophage regulator CSF1. Functional assays containing purified FRCs and monocytes showed that CSF1R signaling was sufficient to support macrophage development. These effects were conserved between mouse and human systems. These data indicate an important role for FRCs in maintaining the LN parenchymal macrophage niche.
Date Issued
2023-09-01
Date Acceptance
2023-03-28
Citation
European Journal of Immunology, 2023, 53 (9)
ISSN
0014-2980
Publisher
Wiley
Journal / Book Title
European Journal of Immunology
Volume
53
Issue
9
Copyright Statement
© 2023 The Authors. European Journal of Immunology published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:001030367100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
ACTIVATION
ANTIGEN
CSF1
DENDRITIC CELLS
Fibroblastic reticular cells
FOLLICLE
HOMEOSTASIS
Human lymph nodes
Immunology
INFECTION
Life Sciences & Biomedicine
Lymph node stromal cells
Macrophages
MONOCYTES
Science & Technology
STIMULATING FACTOR-I
SUBCAPSULAR SINUS MACROPHAGES
TISSUE
Publication Status
Published
Coverage Spatial
Germany
Article Number
2250355
Date Publish Online
2023-09-07