Ceramic hollow fibre constructs for continuous perfusion and cell harvest from 3D hematopoietic organoids
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Tissue vasculature efficiently distributes nutrients, removes metabolites, and possesses selective cellular permeability for tissue growth and function. Engineered tissue models have been limited by small volumes, low cell densities, and invasive cell extraction due to ineffective nutrient diffusion and cell-biomaterial attachment. Herein, we describe the fabrication and testing of ceramic hollow fibre membranes (HFs) able to separate red blood cells (RBCs) and mononuclear cells (MNCs) and be incorporated into 3D tissue models to improve nutrient and metabolite exchange. These HFs filtered RBCs from human umbilical cord blood (CB) suspensions of 20% RBCs to produce 90% RBC filtrate suspensions. When incorporated within 5 mL of 3D collagen-coated polyurethane porous scaffold, medium-perfused HFs maintained nontoxic glucose, lactate, pH levels, and higher cell densities over 21 days of culture in comparison to nonperfused 0.125 mL scaffolds. This hollow fibre bioreactor (HFBR) required a smaller per-cell medium requirement and operated at cell densities > 10-fold higher than current 2D methods whilst allowing for continuous cell harvest through HFs. Herein, we propose HFs to improve 3D cell culture nutrient and metabolite diffusion, increase culture volume and cell density, and continuously harvest products for translational cell therapy biomanufacturing protocols.
Date Issued
2018-04-02
Date Acceptance
2018-01-04
Citation
Stem Cells International, 2018
ISSN
1687-9678
Publisher
Hindawi Publishing Corporation
Journal / Book Title
Stem Cells International
Volume
2018
Copyright Statement
© 2018 Mark C. Allenby et al. This is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor
Commission of the European Communities
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000429639200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Grant Number
340719
Subjects
Science & Technology
Life Sciences & Biomedicine
Cell & Tissue Engineering
Cell Biology
RED-BLOOD-CELLS
ON-A-CHIP
BONE-MARROW
STEM-CELLS
WATER-TREATMENT
WASTE-WATER
IN-VITRO
BIOREACTOR
MEMBRANES
SCAFFOLDS
Publication Status
Published
Article Number
ARTN 6230214