Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Extracellular nucleotide metabolism controls thrombosis and inflammation and may affect degeneration and calcification of aortic valve prostheses. We evaluated the effect of different decellularization strategies on enzyme activities involved in extracellular nucleotide metabolism. Porcine valves were tested intact or decellularized either by detergent treatment or hypotonic lysis and nuclease digestion. The rates of ATP hydrolysis, AMP hydrolysis, and adenosine deamination were estimated by incubation of aorta or valve leaflet sections with substrates followed by HPLC analysis. We demonstrated relatively high activities of ecto-enzymes on porcine valve as compared to the aortic wall. Hypotonic lysis/nuclease digestion preserved >80 % of ATP and AMP hydrolytic activity but reduced adenosine deamination to <10 %. Detergent decellularization completely removed (<5 %) all these activities. These results demonstrate high intensity of extracellular nucleotide metabolism on valve surface and indicate that various valve decellularization techniques differently affect ecto-enzyme activities that could be important in the development of improved valve prostheses.
Date Issued
2016-02-01
Date Acceptance
2016-01-04
Citation
Journal of Cardiovascular Translational Research, 2016, 9 (2), pp.119-126
ISSN
1937-5395
Publisher
Springer Verlag
Start Page
119
End Page
126
Journal / Book Title
Journal of Cardiovascular Translational Research
Volume
9
Issue
2
Copyright Statement
© The Author(s) 2016. This article is published with open access at Springerlink.com.
License URL
Sponsor
The Magdi Yacoub Institute
The Magdi Yacoub Institute
Grant Number
HSC299/12
N/A
Subjects
Science & Technology
Life Sciences & Biomedicine
Cardiac & Cardiovascular Systems
Medicine, Research & Experimental
Cardiovascular System & Cardiology
Research & Experimental Medicine
(Max 10) heart valves
Xenografts
Nucleotide metabolism
ATP
Adenosine
Valve prostheses
HUMAN ENDOTHELIAL-CELLS
HIGH-RISK PATIENTS
HEART-VALVE
ROSS PROCEDURE
PULMONARY HOMOGRAFT
ADENOSINE
PERFORMANCE
REPLACEMENT
1ST
ECTO-5'-NUCLEOTIDASE
Publication Status
Published