Daphnia magna and Xenopus laevis as in vivo models to probe toxicity and uptake of quantum dots functionalized with gH625
Author(s)
Type
Journal Article
Abstract
Abstract: The use of quantum dots (QDs) for nanomedicine is hampered by their potential toxicologic effects and difficulties with delivery into the cell interior. We accomplished an in vivo study exploiting Daphnia magna and Xenopus laevis to evaluate both toxicity and uptake of QDs coated with the membranotropic peptide gH625 derived from the glycoprotein H of herpes simplex virus and widely used for drug delivery studies. We evaluated and compared the effects of QDs and gH625-QDs on the survival, uptake, induction of several responsive pathways and genotoxicity in D. magna, and we found that QDs coating plays a key role. Moreover, studies on X. laevis embryos allowed to better understand their cell/tissue localization and delivery efficacy. X. laevis embryos raised in Frog Embryo Teratogenesis Assay-Xenopus containing QDs or gH625-QDs showed that both nanoparticles localized in the gills, lung and intestine, but they showed different distributions, indicating that the uptake of gH625-QDs was enhanced; the functionalized QDs had a significantly lower toxic effect on embryos’ survival and phenotypes. We observed that D. magna and X. laevis are useful in vivo models for toxicity and drug delivery studies.
Date Issued
2017
Online Publication Date
2024-06-14T11:35:27Z
Date Acceptance
2017-01-26
Citation
International Journal of Nanomedicine, 2017, 12, pp.2717-2731
ISSN
1176-9114
Publisher
Dove Medical Press
Start Page
2717
End Page
2731
Journal / Book Title
International Journal of Nanomedicine
Volume
12
Copyright Statement
© 2017 The Author(s). This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000398662800003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
blood-brain barrier
CELL
CUO
delivery
DELIVERY
DRUG
genotoxicity
Life Sciences & Biomedicine
membranotropic peptide
nanoparticles
NANOPARTICLES
Nanoscience & Nanotechnology
PEPTIDE
Pharmacology & Pharmacy
Science & Technology
Science & Technology - Other Topics
SIMPLEX-VIRUS TYPE-1
TIO2
ZEBRAFISH
ZNO
Publication Status
Published
Date Publish Online
2017-04-04