Advances in two-photon scanning and scanless microscopy technologies for functional neural circuit imaging
File(s)
Accepted version
Published version
Author(s)
Schultz, SR
Copeland, CS
Foust, AJ
Quicke, P
Schuck, R
Type
Journal Article
Abstract
Recent years have seen substantial developments in technology for imaging neural circuits, raising the prospect of large-scale imaging studies of neural populations involved in information processing, with the potential to lead to step changes in our understanding of brain function and dysfunction. In this paper, we will review some key recent advances: improved fluorophores for single-cell resolution functional neuroimaging using a two-photon microscope; improved approaches to the problem of scanning active circuits; and the prospect of scanless microscopes which overcome some of the bandwidth limitations of current imaging techniques. These advances in technology for experimental neuroscience have in themselves led to technical challenges, such as the need for the development of novel signal processing and data analysis tools in order to make the most of the new experimental tools. We review recent work in some active topics, such as region of interest segmentation algorithms capable of demixing overlapping signals, and new highly accurate algorithms for calcium transient detection. These advances motivate the development of new data analysis tools capable of dealing with spatial or spatiotemporal patterns of neural activity that scale well with pattern size.
Date Issued
2016-09-28
Date Acceptance
2016-06-02
Citation
Proceedings of the IEEE, 2016, 105 (1), pp.139-157
ISSN
0018-9219
Publisher
IEEE
Start Page
139
End Page
157
Journal / Book Title
Proceedings of the IEEE
Volume
105
Issue
1
Copyright Statement
This work is licensed under a Creative Commons Attribution 3.0 License. For more information, see http://creativecommons.org/licenses/by/3.0/
Sponsor
The Royal Society
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Cou
Commission of the European Communities
Biotechnology and Biological Sciences Research Council (BBSRC)
The Michael Uren Foundation
GlaxoSmithKline Services Unlimited
Royal Academy Of Engineering
National Institutes of Health
Grant Number
IF110059
BB/K001817/1
512867101
289146
BB/L018268/1
N/A
4124843
RF1415\14\26
UPMC: C15/0244
Subjects
Science & Technology
Technology
Engineering, Electrical & Electronic
Engineering
Acousto-optic
beamlets
calcium imaging
calcium transient detection
cortical circuits
galvanometric scanning
holography
lightsheet microscopy
multiphoton imaging
neural code
neurotechnology
MULTIFOCAL MULTIPHOTON MICROSCOPY
LIGHT-SHEET MICROSCOPY
INDEPENDENT COMPONENT ANALYSIS
NEOCORTICAL PYRAMIDAL NEURONS
SELECTIVE PLANE ILLUMINATION
ENCODED VOLTAGE INDICATORS
GREEN FLUORESCENT PROTEIN
SKELETAL-MUSCLE FIBERS
MOUSE VISUAL-CORTEX
FIELD-OF-VIEW
acousto-optic
light sheet microscopy
0801 Artificial Intelligence And Image Processing
0903 Biomedical Engineering
0906 Electrical And Electronic Engineering
Publication Status
Published