Host transcriptomic response following administration of rotavirus vaccine in infants' mimics wild type infection
File(s)
Published version
Author(s)
Gómez-Carballa, Alberto
Barral-Arca, Ruth
Cebey-López, Miriam
Currás-Tuala, Maria José
Pischedda, Sara
Type
Journal Article
Abstract
Background: Rotavirus (RV) is an enteric pathogen that has devastating impact on childhood morbidity and mortality worldwide. The immunologic mechanism underlying the protection achieved after RV vaccination is not yet fully understood. Methods: We compared the transcriptome of children affected by community-acquired RV infection and children immunized with a live attenuated RV vaccine (RotaTeq®). Results: RV vaccination mimics the wild type infection causing similar changes in children's transcriptome, including transcripts associated with cell cycle, diarrhea, nausea, vomiting, intussusception, and abnormal morphology of midgut. A machine learning approach allowed to detect a combination of nine-transcripts that differentiates vaccinated from convalescent-naturally infected children (AUC: 90%; 95%CI: 70-100) and distinguishes between acute-infected and healthy control children (in both cases, AUC: 100%; 95%CI: 100-100). We identified a miRNA hsa-mir-149 that seems to play a role in the host defense against viral pathogens and may have an antiviral role. Discussion: Our findings might shed further light in the understanding of RV infection, its functional link to intussusception causes, as well as guide development of antiviral treatments and safer and more effective vaccines. The nine-transcript signature may constitute a marker of vaccine protection and helps to differentiate vaccinated from naturally infected or susceptible children.
Date Issued
2021-01-21
Date Acceptance
2020-12-02
Citation
Frontiers in Immunology, 2021, 11
ISSN
1664-3224
Publisher
Frontiers Media
Journal / Book Title
Frontiers in Immunology
Volume
11
Copyright Statement
© 2021 Gómez-Carballa, Barral-Arca, Cebey-López, Currás-Tuala, Pischedda, Gómez-Rial, Habgood-Coote, Herberg, Kaforou, Martinón-Torres and Salas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Sponsor
Wellcome Trust
European Commission
European Commission
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/33552046
Grant Number
206508/Z/17/Z
Horizon 2020
279185
Subjects
RNA-seq
biomarkers
intussusception
machine learning
miRNA
rotavirus
transcriptomics
vaccination
Publication Status
Published
Coverage Spatial
Switzerland
Article Number
ARTN 580219