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Immunohistochemical analysis of expression, phosphorylation, and nuclear translocation of NF-κB proteins in human tissues
File(s)
Author(s)
Vecchiotti, Davide
Verzella, Daniela
Capece, Daria
Cornice, Jessica
Nolfi, Mauro Di Vito
Type
Journal Article
Abstract
Immunohistochemistry (IHC) is a technique aimed at detecting specific antigens on tissue sections by the use of targeting reagents labeled with reporter molecules. This technique allows a snapshot of the structure of tissue and determines the cellular and subcellular localization of a target antigen. This chapter describes how to identify and localize NF-κB proteins in human tissue using immunohistochemical staining on formalin-fixed paraffin-embedded and frozen tissue.
Date Issued
2021-07-09
Date Acceptance
2021-01-01
Citation
Methods in Molecular Biology, 2021, 2366, pp.27-42
URI
DOI
ISSN
1064-3745
Publisher
Humana Press
Start Page
27
End Page
42
Journal / Book Title
Methods in Molecular Biology
Volume
2366
Copyright Statement
© Springer Science+Business Media, LLC, part of Springer Nature 2021. The final publication is available at Springer via https://link.springer.com/protocol/10.1007/978-1-0716-1669-7_3
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/34236631
Subjects
Antibodies
Antigen retrieval
Detection
Fixation
Identification (localization)
Microscope
NF-κB
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2021-07-09