New HPLC method for determination of cystatin C biomarker in human blood
File(s)
Published version
Author(s)
AlMusaimi, Othman Ibrahem
Abu-Nawwas, Abd-Alhakeem Hasan
AlShaer, Danah Mahdi
Khaleel, Nabeel Yousef
Type
Journal Article
Abstract
A rapid and sensitive method for determination of cystatin C (CC) protein in human blood by means of high performance liquid chromatography (HPLC) was developed and validated. Estimated glomerular filtration rate (eGFR) has been calculated for the patients with chronic kidney disease (CKD). Ion pair liquid chromatography technique was utilized to separate CC along with UV detection. Calibration curve with excellent correlation (r2 = 0.99) over the range from 0.75 to 20.50 mg/L of CC was accomplished. Limits of detection and quantify-cation were 0.375 and 0.75 mg/L of CC, respectively. The recoveries were in the range of 93.6-102.3%. Intra-assay and inter-assay variabilities were 8.2 and 6.14%, respectively.
Date Acceptance
2017-10-25
Citation
European Journal of Chemistry, 8 (4), pp.378-383
ISSN
2153-2249
Publisher
European Journal of Chemistry
Start Page
378
End Page
383
Journal / Book Title
European Journal of Chemistry
Volume
8
Issue
4
Copyright Statement
© 2017 Atlanta Publishing House LLC - All rights reserved
Sponsor
King Abdulaziz City for Science and Technology (KACST)
Identifier
https://www.eurjchem.com/index.php/eurjchem/article/view/1658
Grant Number
SP36-16
Publication Status
Published
Date Publish Online
2017-12-31