Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Context: Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone
that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been
studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative
correlation between its term placental expression and birth weight.
Objective: The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and
IGF2R, and PHLDA2 on term birth weight.
Design: Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in
chorionic villus samples (CVS) (n = 260) collected at 11–13 weeks’ gestation. Expression was correlated with term birth
weight using statistical package R including correction for several confounding factors.
Results: Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04,
respectively). No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA)
neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3?661027
).
Interpretation: Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future
fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This
research reveals an imprinted, parentally driven rheostat for in utero growth.
that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been
studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative
correlation between its term placental expression and birth weight.
Objective: The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and
IGF2R, and PHLDA2 on term birth weight.
Design: Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in
chorionic villus samples (CVS) (n = 260) collected at 11–13 weeks’ gestation. Expression was correlated with term birth
weight using statistical package R including correction for several confounding factors.
Results: Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04,
respectively). No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA)
neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3?661027
).
Interpretation: Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future
fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This
research reveals an imprinted, parentally driven rheostat for in utero growth.
Date Issued
2014-01-15
Date Acceptance
2013-12-04
Citation
PLoS ONE, 2014, 9 (1)
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Journal / Book Title
PLoS ONE
Volume
9
Issue
1
Copyright Statement
© 2014 Demetriou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000330235100089&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
FOR-GESTATIONAL-AGE
IGF-I RECEPTOR
FETAL-GROWTH
CORD-BLOOD
PLACENTAL EXPRESSION
PHLDA2 GENE
INTRAUTERINE
RESTRICTION
NEWBORNS
RETARDATION
Publication Status
Published
Article Number
e85454
Date Publish Online
2014-01-15